Trine Eker Christoffersen

• Grønlien, Heidi Kristine; Christoffersen, Trine Eker; Ringstad, Øystein; Andreassen, Marita & Lugo, Ricardo Gregorio (2021). A blended learning teaching strategy strengthens the nursing students’ performance and self-reported learning outcome achievement in an anatomy, physiology and biochemistry course: A quasi-experimental study. Nurse Education in Practice. ISSN 1471-5953. 52, p. 1–6. doi: 10.1016/j.nepr.2021.103046. Full text in Research Archive Show summary

  In nursing, bioscience is regarded as one of the cornerstones of nursing practice. However, bioscience disciplines as anatomy, physiology and biochemistry are considered challenging for students and the failure rate is high. In this study we explore a blended learning teaching strategy in an anatomy, physiology and biochemistry course for first year Bachelor nursing students. In the blended learning teaching strategy, short narrated online digital resources of bioscientific terms and concepts were integrated into the teaching design along with digital metacognitive evaluations of learning outcomes. Results show that compared to students receiving traditional face-to-face teaching, the students with a blended learning approach performed better on their national exam with a small to medium effect size (Cohen’s d=0.23). Student course evaluations supported the blended learning delivery with small to medium effect sizes. The students reported that the digital resources supported their learning outcome achievement, that they better understood the teacher’s expectations and that they were more satisfied with their virtual learning environment. This study adds to the growing literature of blended learning effectiveness in higher education, and suggests the use of digital resources as an enrichment of teaching and enhancement of students’ study experience.

• Grønlien, Heidi Kristine; Christoffersen, Trine Eker; Furlund, Camilla Bø; Garabet, Lamya Samir Noori; Syvertsen, Terje & Moe, Morten Kaare [Show all 8 contributors for this article] (2020). Cytokine and gene expression profiling in patients with HFE-associated hereditary hemochromatosis according to genetic profile. Acta Haematologica. ISSN 0001-5792. doi: 10.1159/000511551. Full text in Research Archive Show summary

  Background: Hemochromatosis gene (HFE)-associated hereditary hemochromatosis (HH) is characterized by downregulation of hepcidin synthesis, leading to increased intestinal iron absorption. Objectives: The objectives were to characterize and elucidate a possible association between gene expression profile, hepcidin levels, disease severity, and markers of inflammation in HFE-associated HH patients. Methods: Thirty-nine HFE-associated HH patients were recruited and assigned to 2 groups according to genetic profile: C282Y homozygotes in 1 group and patients with H63D, as homozygote or in combination with C282Y, in the other group. Eleven healthy first-time blood donors were recruited as controls. Gene expression was characterized from peripheral blood cells, and inflammatory cytokines and hepcidin-25 isoform were quantified in serum. Biochemical disease characteristics were recorded. Results: Elevated levels of interleukin 8 were observed in a significant higher proportion of patients than controls. In addition, compared to controls, gene expression of ζ-globin was significantly increased among C282Y homozygote patients, while gene expression of matrix metalloproteinase 8, and other neutrophil-secreted proteins, was significantly upregulated in patients with H63D. Conclusion: Different disease signatures may characterize HH patients according to their HFE genetic profile. Studies on larger populations, including analyses at protein level, are necessary to confirm these findings.

• Bui, Tri Quang; Cao, Vinh Duy; Do, Nu Bich Duyen; Christoffersen, Trine Eker; Wang, Wei & Kjøniksen, Anna-Lena (2018). Salinity gradient energy from expansion and contraction of poly(allylamine hydrochloride) hydrogels. ACS Applied Materials & Interfaces. ISSN 1944-8244. 10(26), p. 22218–22225. doi: 10.1021/acsami.8b05333. Full text in Research Archive Show summary

  Salinity gradients exhibit a great potential for production of renewable energy. Several techniques such as pressure-retarded osmosis and reverse electrodialysis have been employed to extract this energy. Unfortunately, these techniques are restricted by the high costs of membranes and problems with membrane fouling. However, the expansion and contraction of hydrogels can be a new and cheaper way to harvest energy from salinity gradients since the hydrogels swell in freshwater and shrink in saltwater. We have examined the effect of cross-linker concentration and different external loads on the energy recovered for this type of energy-producing systems. Poly(allylamine hydrochloride) hydrogels were cross-linked with glutaraldehyde to produce hydrogels with excellent expansion and contraction properties. Increasing the cross-linker concentration markedly improved the energy that could be recovered from the hydrogels, especially at high external loads. A swollen hydrogel of 60 g could recover more than 1800 mJ when utilizing a high cross-linker concentration, and the maximum amount of energy produced per gram of polymer was 3.4 J/g. Although more energy is recovered at high cross-linking densities, the maximum amount of energy produced per gram of polymer is highest at an intermediate cross-linking concentration. Energy recovery was reduced when the salt concentration was increased for the low-concentration saline solution. The results illustrate that hydrogels are promising for salinity gradient energy recovery, and that optimizing the systems significantly increases the amount of energy that can be recovered.

• Sveen, Ole; Skaug, Arne; Christoffersen, Trine Eker; Jensen, Jørgen & Hanssen, Knut Egil (2017). A combined and intensive exercise model improves physical capacity in cardiac patients. Journal of Clinical Trials. 7(5). doi: 10.4172/2167-0870.1000326. Full text in Research Archive Show summary

  Objective: We have measured the effect of combined strength and high intensity endurance training on physical capacity and lipid profile in patients with heart and coronary diseases. We wanted to see whether cardiac patients were able to increase training intensity to improve strength without losing endurance capacity. Methods: Thirty heart-operated subjects participated in an intervention period of 10 weeks. The age of the participants was between 52-72 years. The Resistance-Interval group (RE-INT) practiced four times a week with two intensive endurance (spinning) sessions and two strength training sessions. The endurance training consisted of intervals where the heart rate reached>90% of maximum heart rate. Strength training was performed in three series with a load of 8-12 repetition maximum (RM). The subjects in the control group (CON) performed two to three sessions per week according to a national program specialized for coronary patients (called “Ullevaal model”). We had a randomized controlled trial. Results: Maximal leg strength increased in both groups during the intervention, but the increase was higher in the RE-INT group (from 107.9 ± 8.1 kg to 162.0 ± 8.4 kg) compared to the CON group (from 110.8 ± 8.9 to 125.4 ± 9.5 ) (p<0.001). Strength in chest press, the maximal oxygen uptake and the concentration of high densitylipoprotein (HDL) protein increased in both groups during the test period. However, no differences were observed between the RE-INT and CON group. Low density-lipoprotein (LDL), blood pressure and body weight did not change during the intervention period in any of the groups. Conclusion: cardiac patients were able to increase training intensity, strength and maximal oxygen uptake during a period of 10 weeks. We found that combined training has an effective impact on the increase in leg strength. The increase in muscle strength can be vital for the everyday quality of life in cardiac patients.

• Solhaug, Anita; Wisbech, Cathrine; Christoffersen, Trine Eker; Hult, Lene Therese Olsen; Lea, Tor Erling & Eriksen, Gunnar Sundstøl [Show all 7 contributors for this article] (2015). The mycotoxin alternariol induces DNA damage and modify macrophage phenotype and inflammatory responses. Toxicology Letters. ISSN 0378-4274. 239(1), p. 9–21. doi: 10.1016/j.toxlet.2015.08.1107. Full text in Research Archive
• Christoffersen, Trine Eker; Hult, Lene Therese Olsen; Solberg, Henriette; Bakke, Anne Marie; Kuczkowska, Katarzyna & Huseby, Eirin Cathrine [Show all 9 contributors for this article] (2015). Effects of the non-commensal Methylococcus capsulatus Bath on mammalian immune cells. Molecular Immunology. ISSN 0161-5890. 66(2), p. 107–116. doi: 10.1016/j.molimm.2015.02.019. Full text in Research Archive
• Christoffersen, Trine Eker; Hult, Lene Therese Olsen; Kuczkowska, Katarzyna; Moe, Kim Marius; Skeie, Siv Borghild & Lea, Tor Erling [Show all 7 contributors for this article] (2014). In Vitro Comparison of the Effects of Probiotic, Commensal and Pathogenic Strains on Macrophage Polarization. Probiotics and Antimicrobial Proteins. ISSN 1867-1306. 6(1), p. 1–10. doi: 10.1007/s12602-013-9152-0. Full text in Research Archive
• Christoffersen, Trine Eker; Larsen, Astrid Lervik; Andresen, Cathrine; Naseem, Khushbakht; Rahhaoui, Mariam & Johannessen, Pernille (2014). Underdiagnostiseres Giardia lamblia og Cryptosporidium med nåværende rutinemetode for parasittundersøkelse? Bioingeniøren. ISSN 0801-6828. p. 20–22. Full text in Research Archive
• Kleiveland, Charlotte Ramstad; Hult, Lene Therese Olsen; Spetalen, Signe; Kaldhusdal, Magne; Christoffersen, Trine Eker & Bengtsson, Oskar [Show all 9 contributors for this article] (2013). The Noncommensal Bacterium Methylococcus capsulatus (Bath) Ameliorates Dextran Sulfate (Sodium Salt)-Induced Ulcerative Colitis by Influencing Mechanisms Essential for Maintenance of the Colonic Barrier Function. Applied and Environmental Microbiology. ISSN 0099-2240. 79(1), p. 48–56. doi: 10.1128/AEM.02464-12. Full text in Research Archive
• Christoffersen, Trine Eker; Jensen, Hanne; Kleiveland, Charlotte Ramstad; Dørum, Guro; Jacobsen, Morten & Lea, Tor Erling (2012). In vitro comparison of commensal, probiotic andpathogenic strains of Enterococcus faecalis. British Journal of Nutrition. ISSN 0007-1145. 108(11), p. 2043–2053. doi: 10.1017/S0007114512000220. Full text in Research Archive Show summary

  In vivo studies have provided evidence that micro-organisms have important roles in immunological, digestive and respiratory functions, conferring health benefits on the host. Several in vitro methods have been advised for the initial screening of microbes with potential health effects. The objective of the present study was to employ such in vitro methodology to characterise different strains of Enterococcus faecalis. The characteristics of a commercial product marketed as a probiotic, Symbioflor-1 (Symbiopharm), were compared with the characteristics of both pathogenic and commensal strains. Tolerance towards low pH and viability after exposure to human gastric and duodenal juices were assayed. Symbioflor-1 was the most susceptible strain to these treatments when compared with the other E. faecalis strains. Furthermore, Symbioflor-1 exhibited the lowest adhesion capacity to intestinal epithelial cells (IEC) and mucus. Competitive binding studies using heparin indicated that glycosaminoglycans might be involved in the adhesion to IEC, but also that differences in these putative bacteria-host interactions do not cause the relative low adhesion capacity of Symbioflor-1. Maturation of dendritic cells (DC) after exposure to bacteria was assayed as an indication of an immunomodulatory effect. All strains induced a moderate elevation of the DC maturation markers CD83 and CD86; however, no strain-specific differences were detected. Correlations between in vitro and in vivo studies are discussed. Although in vitro assaying is a rational starting point for the selection of microbes with a potential health benefit, it is emphasised that human clinical trials are the definite tool for establishing probiotic status.

• Sørensen, Vigdis; Zhen, Yan; Zakrzewska, Malgorzata; Haugsten, Ellen Margrethe; Walchli, S & Nilsen, Trine [Show all 8 contributors for this article] (2008). Phosphorylation of fibroblast growth factor (FGF) receptor 1 at Ser777 by p38 mitogen-activated protein kinase regulates translocation of exogenous FGF1 to the cytosol and nucleus. Molecular and Cellular Biology. ISSN 0270-7306. 28(12), p. 4129–4141. doi: 10.1128/MCB.02117-07. Full text in Research Archive
• Nilsen, Trine; Rosendal, Ken; Sørensen, Vigdis; Wesche, Jørgen; Olsnes, Sjur & Widocha, Antoni (2007). A nuclear export sequence located on a beta-strand in fibroblast growth factor-1. Journal of Biological Chemistry. ISSN 0021-9258. 282(36), p. 26245–26256. doi: 10.1074/jbc.M611234200. Full text in Research Archive
• Sørensen, Vigdis; Nilsen, Trine & Wiedlocha, Antoni (2006). Functional diversity of FGF-2 isoforms by intracellular sorting. Bioessays. ISSN 0265-9247. 28. Full text in Research Archive
• Wiedlocha, Antoni; Nilsen, Trine; Wesche, Jørgen; Sørensen, Vigdis; Malecki, M & Marcinkowska, Ewa [Show all 7 contributors for this article] (2005). Phosphorylation-regulated nucleocytoplasmic trafficking of internalized fibroblast growth factor-1. Molecular Biology of the Cell. ISSN 1059-1524. p. 794–810. Full text in Research Archive
• Wesche, Jørgen; Malecki, Jedrzej M.; Wiedlocha, Antoni; Ehsani, Maryam; Nilsen, Trine & Marcinkowska, Ewa [Show all 7 contributors for this article] (2005). Two nuclear localization signals required for transport from the cytosol to the, nucleus of externally added FGF-1 translocated into cells. Biochemistry (Moscow). ISSN 0006-2979. 44. Full text in Research Archive
• Christoffersen, Trine Eker (2003). Phosphorylation of insulin-like growth factor binding protein-3 by deoxyribonucleic acid-dependent protein kinase reduces ligand binding and enhances nuclear accumulation. Endocrinology. ISSN 0013-7227. 144(5), p. 1984–1993. doi: 10.1210/en.2002-220798. Full text in Research Archive

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• Celis, Julio E. & Christoffersen, Trine Eker (2006). Cell biology, A Laboratory handbook. Chapter 35. Peroxisomal targeting as a tool to assess protein-protein interactions. Elsevier. ISBN 9780121647315. Full text in Research Archive

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• Bergsjø, Leonora Onarheim; Leonardsen, Ann-Chatrin Linqvist; Heggernes, Pinar; Said, Fahad Faisal; Engeness, Irina & Christoffersen, Trine Eker (2025). Kunstig eller intelligent? Høyere utdanning i identitetskrise. doi: https:/www.arendalsuka.no/programsoek/details/28499. Full text in Research Archive
• Christoffersen, Trine Eker & Fekjær, Silje (2024). Leder eller fagperson. På tide å eie rollen vår. Full text in Research Archive
• Christoffersen, Trine Eker (2017). Lær om kroppen - hvor som helst, når som helst. Full text in Research Archive
• Christoffersen, Trine Eker (2017). Pilotprosjekt for digitalisering av undervisning - Hva er status? Full text in Research Archive
• Christoffersen, Trine Eker; Grønlien, Heidi Kristine & Dahl, Mette Lundstrøm (2017). Bruk av skjermopptak i undervsining. Full text in Research Archive
• Christoffersen, Trine Eker (2016). Ja til møkkaunger. [Journal]. Verdens Gang (VG), VG Helg. Full text in Research Archive
• Christoffersen, Trine Eker (2015). RNA; mer enn m-, t- og rRNA. Full text in Research Archive
• Christoffersen, Trine Eker; Hult, Lene Therese Olsen; Kleiveland, Charlotte Ramstad & Lea, Tor Erling (2014). Bakteriers påvirkning på immunceller. Full text in Research Archive
• Christoffersen, Trine Eker (2014). Den viktige mikrofloraen. Full text in Research Archive
• Christoffersen, Trine Eker (2014). Samlivet med bakterier viktig for helsen. p. 8–9. Full text in Research Archive
• Solhaug, Anita; Wisbech, Cathrine; Christoffersen, Trine Eker; Hult, Lene Therese Olsen; Lea, Tor Erling & Vines, Laura [Show all 9 contributors for this article] (2013). Alternariol induces differentiation of macrophages. Full text in Research Archive
• Christoffersen, Trine Eker & Larsen, Astrid Lervik (2013). Er parasittinfeksjoner underdiagnostisert i Østfold? Full text in Research Archive
• Christoffersen, Trine Eker (2012). Er in vitro testing tilstrekkelig for å gi mikrober probiotisk status? Full text in Research Archive
• Christoffersen, Trine Eker (2012). In vitro testing av tarmbakteriers probiotiske egenskaper. Full text in Research Archive
• Christoffersen, Trine Eker (2012). Assaying probiotic properties of Enterococcus faecalis strains. Full text in Research Archive
• Christoffersen, Trine Eker (2012). Tarmforskning vekker oppsikt. [Journal]. Fredrikstad blad. Full text in Research Archive
• Christoffersen, Trine Eker (2012). Tøffere tider for helsepåstander. p. 8–9. Full text in Research Archive
• Kleiveland, Charlotte Ramstad; Hult, Lene Therese Olsen; Spetalen, Signe; Kaldhusdal, Magne; Christoffersen, Trine Eker & Bengtsson, Oskar [Show all 9 contributors for this article] (2012). protective role of a non-commensal methanotroph bacterium in a mouse model of colitis. Full text in Research Archive
• Christoffersen, Trine Eker; Kleiveland, Charlotte Ramstad; Hult, Lene Therese Olsen; Jacobsen, Morten & Lea, Tor Erling (2012). In vitro methods for establishing probiotic status, not sufficient when comparing Enterococcus faecalis strains. Full text in Research Archive
• Christoffersen, Trine Eker (2012). Tarmbakterier - hvorfor er det interessant? Full text in Research Archive
• Christoffersen, Trine Eker; Kleiveland, Charlotte Ramstad; Hult, Lene Therese Olsen; Jacobsen, Morten & Lea, Tor Erling (2012). Bakteriers effekt på inflammatorisk tarmsykdom i mus. Full text in Research Archive
• Christoffersen, Trine Eker (2011). Probiotiske bakterier; fleip eller fakta? Full text in Research Archive
• Christoffersen, Trine Eker (2011). Helsebringende bakterier; fleip eller fakta? Full text in Research Archive
• Christoffersen, Trine Eker (2010). Bakterie-verts interaksjoner i tarmen. Functional consequences of bacteria-host interactions in the intestine. Full text in Research Archive
• Christoffersen, Trine Eker (2009). Functional consequenses of bacterial-host interactions in the intestinal epithelium. Full text in Research Archive
• Christoffersen, Trine Eker (2009). Funksjonelle konsekvenser av bakterie-vert-interaksjoner i tarmen. Full text in Research Archive Show summary

  Foredrag ved Forskningsdagene

• Christoffersen, Trine Eker (2009). Functional consequences of bacteria-host interaction in the intestine. Full text in Research Archive
• Christoffersen, Trine Eker (2009). Ko-kultur design. Full text in Research Archive
• Grønlien, Heidi Kristine & Christoffersen, Trine Eker (2009). Da damene dro hjem. Full text in Research Archive Show summary

  Presentasjon av unge, kvinnelige førsteamanuensiser på HiØ og våre forskningsprosjekt. For Heidi Grønlien ble prosjektet om pubertetsutvikling i torsk presentert på en humørfylt og publikumsforståelig måte.

• Huseby, Eirin; Lea, Tor Erling; Kleiveland, Charlotte Ramstad; Christoffersen, Trine Eker & Hult, Lene Therese Olsen (2013). Dendrittiske celler og Methylococcus capsulatus (Bath)interaksjon og funksjonelle egenskaper. UMB. Full text in Research Archive
• Linn, Floer; Christoffersen, Trine Eker; Kleiveland, Charlotte Ramstad; Hult, Lene Therese Olsen & Lea, Tor Erling (2012). Methylococcus capsulatus og interaksjoner med humane monocytter og monocytt-deriverte dendrittiske celler. UMB. Full text in Research Archive
• Lea, Tor Erling; Hult, Lene Therese Olsen; Gillesen, Lillian; Kleiveland, Charlotte Ramstad & Christoffersen, Trine Eker (2011). Probiotika og interaksjon med dendrittiske celler og tarmepitel. Norges miljø- og biovitenskapelige universitet (NMBU). Full text in Research Archive Show summary

  Fordøyelsessystemet utgjør en elementær viktighet for vår helse og velvære. Tarmoverflaten består av et enkelt lag med epitelceller som skiller innholdet i tarmlumen fra kroppens andre vev. I tarmen finnes det ulike typer celler, som blant annet står for absorpsjon av næringsstoffer. Tarmepitelet blir hele tiden utsatt for ulike mikroorganismer, både gjennom tarmens egne bakterier og gjennom kosten som inntas. Immunsystemet må skille mellom eget og fremmed, og mellom fredelige og sykdomsfremkallende mikrober for å unngå sykdommer som matallergi, kronisk inflammatorisk tarmsykdom og tarmkreft. Dendrittiske celler har svært viktige funksjoner ved igangsettelse av immunrespons i tarm. Dendrittene på de dendrittiske cellene kan forlenges og trenge mellom epitelcellene slik at de kommer i direkte kontakt med innholdet i tarmlumen. Dendrittiske celler vil kjenne igjen fremmede mikroorganismer. De vil da modnes og migrere til lymfeknuter, hvor de vil stimulere T-celler. Man ville i denne studien undersøke om dendrittiske celler eksponert for patogene, probiotiske og kommensale Enterococcus faecalis stammer, induserte til ulik ekspresjon av overflatemolekylene og modningsmarkørene CD80 og CD83. De ulike E. faecalis stammene viste ingen forskjeller i evnen til å modne dendrittiske celler. Kondisjonert medium fra dendrittiskeceller eksponert for probiotiske, kommensale og patogene bakterier ble påført den basolaterale siden av polarisert tarmepitel. Tidligere resultater fra en transkriptomanalyse (mikromatrise) hadde vist en kraftig nedregulering av fibrinogen. Man ville verifisere dette ved å bruke real time-PCR, men kunne ikke finne noen markant regulering av uttrykt fibrinogen. The gastrointestinal tract has a fundamental impact on our health and well-being. The intestinal surface consists of a simple layer of epithelial cells that separates the intestinal luminal content from the other tissues in the body. In the intestine, you can find many different cell types that among other things are responsible for absorption of nutrition. The intestinal epithelium is continuously exposed to different microorganisms, both through the intestines own bacteria and trough food ingested. The immune system have to segregate between its own and foreign material, and between unharmful and disease promoting microorganisms to avoid diseases like food allergy, intestinal bowel disease and intestinal cancer. Dendritic cells have a central function in the initiation of an immune response in the intestine. The dendrites on the dendritic cells can extend between the epithelial cells, to get in contact with the content of the intestinal lumen. Dendritic cells will recognize foreign microorganisms and subsequently mature and migrate to lymph nodes, where they will stimulate T-cells. In this study, we wanted to investigate if dendritic cells exposed to a pathogen, a probiotic and a commensal bacteria strain, all E. faecalis strains, induced to differences in the expressions of the surface molecules and maturation markers CD80 and CD83. The different E. faecalis strains didn’t show any differences in their ability to mature dendritic cells. Conditioned medium from dendritic cells exposed to probiotic, commensal and pathogen bacteria strains, was applied on the basolateral side of the polarized intestinal epithelium. Earlier results from a transcriptome analysis (microarray) had shown a strong down-regulation of fibrinogen. We wanted to verify this by using real time-PCR, but could not find any pronounced regulations of expressed fibrinogen.

• Nilsen, Trine & Olsnes, Sjur (2007). Intracellular interactions and nucleocytoplasmic trafficking of exogenous FGF-1. Universitetet i Oslo. ISSN 9788280724403. Full text in Research Archive

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