Beathe Kiland Granerud

• Kildal, Anders Benjamin; Ueland, Thor; Mirlashari, Mohammad Reza; Nissen-Meyer, Lise Sofie; Granerud, Beathe Kiland & Riise, Anne Margarita Dyrhol [Vis alle 44 forfattere av denne artikkelen] (2026). Serial blood serum measurements of calprotectin and deoxyribonuclease in COVID-19 patients during hospitalization and recovery until one year: a prospective, multicenter, observational study. BMC Infectious Diseases. 26. doi: 10.1186/s12879-026-12939-x. Fulltekst i vitenarkiv Vis sammendrag

  Objectives: To examine circulating calprotectin and deoxyribonuclease (DNase) serum levels during hospitalization in COVID-19 patients, their continuity post-discharge and dynamics in relation to disease severity. Methods: Serum levels were measured in samples collected from 252 COVID-19 patients during hospitalization (admission, days 3–5 and 7–10), at 3 months and after 1 year, and related to admission to intensive care unit (ICU) or high dependency unit (HDU), and 60-day total mortality. Results: During hospitalization, calprotectin and DNase levels were significantly elevated compared to healthy controls (HC). Calprotectin was increased in ICU/HDU patients compared to those at wards during hospitalization and in non-survivors compared to survivors during prolonged hospitalization. High calprotectin levels at admission were associated with male sex, PaO2/FiO2 ratio and national COVID-19 wave 2 and 3 compared to wave 1. High admission levels of both calprotectin and ferritin were associated with an approximately twofold higher odds ratio for ICU/HDU admission than either marker alone. DNase was lower at admission in non-survivors compared to survivors. Post-discharge, DNase but not calprotectin levels remained elevated extending to 1-year follow-up compared to HC. Conclusions: COVID‑19 non‑survivors showed persistently higher calprotectin levels during prolonged hospitalization, and elevated admission calprotectin was associated with later pandemic waves and poorer oxygenation. Incorporating calprotectin with ferritin improved prediction of ICU/HDU admission. DNase levels were lower at admission in non‑survivors, while overall DNase remained elevated from hospitalization through 1‑year follow‑up compared with HC, suggesting a possible role in long‑term COVID‑19–related immune alterations.

• Otterdal, Kari; Ueland, Thor; Holter, Jan Cato; Kaarbø, Mari; Schanke, Ylva & Granerud, Beathe Kiland [Vis alle 18 forfattere av denne artikkelen] (2026). Platelet-derived mediators in hospitalized COVID-19 patients and associations to respiratory failure, ICU admittance and 60-day mortality. Frontiers in Cardiovascular Medicine. 13. doi: 10.3389/fcvm.2026.1685861. Fulltekst i vitenarkiv Vis sammendrag

  Background: Platelet activation is documented in COVID-19, but data on platelet-derived mediators are scarce. Objective: To examine the levels of various platelet-derived mediators in relation to adverse outcomes defined as the need for treatment at intensive care unit (ICU) and/or respiratory failure (RF) and 60-day total mortality in hospitalized COVID-19 patients. Methods: Plasma levels of RANTES/CCL5, PF4/CXCL4, ENA78/CXCL5, NAP-2/CXCL7, SDF-1/CXCL12, P-selectin, soluble CD40 ligand (sCD40L) and vascular endothelial cell growth factor A (VEGF-A) were measured in 245 hospitalized COVID-19 patients and in a subpopulation of the patients, also at three, six and 12 months after hospitalization. Results: Our main findings were: (i) High levels of P-selectin was associated with ICU/RF, while low levels of PF4/CXCL4, ENA-78/CXCL5 and NAP-2/CXCL7 were associated with 60-days mortality. (ii) Most of the mediators were normalized after hospitalization, but plasma levels of sCD40L, ENA-78/CXCL7 and VEGF-A were markedly elevated compared to healthy controls for up to 12 months after hospitalization. (iii) In vitro, inactivated SARS-CoV-2 induced a dose-dependent release of NAP-2, P-selectin, RANTES, sCD40L and VEGF-A from isolated platelets. Conclusion: Our findings underscore the role of platelet-derived inflammatory mediators in the pathogenesis of COVID-19, potentially involving direct effects of SARS-CoV-2. The study also points to a persistent platelet activation following hospitalization.

• Stenseth, Else Berit; Syversen, Linda; Andersen, Heidi; Wergeland, Line; Granerud, Beathe Kiland & Ersvær, Elisabeth (2025). Ekstern praksis: Samarbeid og kommunikasjon for kvalitet. Bioingeniøren. ISSN 0801-6828. 8 2025, s. 20–25. Fulltekst i vitenarkiv Vis sammendrag

  Bakgrunn: Eksterne praksisstudier er viktig for studentens profesjonsutvikling. Høy kvalitet på praksisstudiene forutsetter at kontakten mellom praksisstedet og utdanningen er systematisk etablert. I denne studien har vi gjennomført en kartlegging av kommunikasjonsmønstre, informasjonsflyt og samarbeid mellom utdanningsinstitusjon og praksisfeltet, sett fra perspektivet til tre bioingeniørutdanninger i Norge. Material og metode: Det er utført fokusgruppeintervju av ansatte ved tre ulike bioingeniørutdanninger og ett sykehus, etterfulgt av tematisk kvalitativ innholdsanalyse av datamaterialet. Resultat: Undersøkelsen av informantenes erfaringer viser (i) manglende avtaler på studieprogram-/instituttnivå, (ii) informasjonsflyt i utdanningsinstitusjonen kan være ulik, muligens grunnet ulik fakultetstilhørighet, (iii) kommunikasjonen mellom utdanning og praksisfelt er kompleks og foregår på ulike nivåer og med ulike kommunikasjonsmønstre, og (iv) det kommuniseres både om fordeling av studenter samt tematisk innhold i praksis. Konklusjon: Denne studien viser at det er variasjon i kommunikasjon fra sted til sted, og at det er mangelfull kommunikasjon både internt i utdanningsinstitusjonene og mellom utdanningsinstitusjon og praksissted.

• Granerud, Beathe Kiland; Kaarbø, Mari; Al-Baldawi, Huda; Heggelund, Lars; Tveita, Anders & Müller, Karl Erik [Vis alle 42 forfattere av denne artikkelen] (2025). Droplet Digital PCR or Real-Time PCR as a Method for Quantifying SARS-CoV-2 RNA in Plasma-Is There a Difference? Viruses. 17(6). doi: 10.3390/v17060772. Fulltekst i vitenarkiv Vis sammendrag

  The aim of this study is to ascertain whether qRT-PCR (reverse transcriptase real-time PCR) or RT-ddPCR (reverse transcriptase digital droplet PCR) is more effective for detecting SARS-CoV-2 RNA (severe acute respiratory syndrome coronavirus 2 RNA) in blood plasma from COVID-19 (coronavirus infectious disease-19) patients. The E-gene of SARS-CoV-2 RNA was quantified using both methods in 128 plasma samples from 70 hospitalized patients, followed by a statistical analysis to compare the sensitivity and concordance between the methods. Out of the 128 samples, 89 yielded consistent results irrespective of the method used, whereas 39 samples showed discrepancies between the two different methods. RT-ddPCR frequently registered higher viral quantities compared to qRT-PCR; however, the results did not demonstrate a clear superiority in sensitivity for RT-ddPCR. Although RT-ddPCR registered higher viral quantities, this study concludes that both methods provide comparable results for detecting SARS-CoV-2 E-gene RNA in plasma.

• Barratt-Due, Andreas; Pettersen, Kristin; Dahl, Tuva Børresdatter; Holter, Jan Cato; Grønli, Renathe Henriksen & Dyrhol-Riise, Anne Ma [Vis alle 20 forfattere av denne artikkelen] (2024). Escalated complement activation during hospitalization is associated with higher risk of 60-day mortality in SARS-CoV-2-infected patients. Journal of Internal Medicine. ISSN 0954-6820. 296(1), s. 80–92. doi: 10.1111/joim.13783. Fulltekst i vitenarkiv Vis sammendrag

  Background: The complement system, an upstream recognition system of innate immunity, is activated upon SARS-CoV-2 infection. To gain a deeper understanding of the extent and duration of this activation, we investigated complement activation profiles during the acute phase of COVID-19, its persistence post-recovery and dynamic changes in relation to disease severity. Methods: Serial blood samples were obtained from two cohorts of hospitalized COVID-19 patients (n = 457). Systemic complement activation products reflecting classical/lectin (C4d), alternative (C3bBbP), common (C3bc) and terminal pathway (TCC and C5a) were measured during hospitalization (admission, days 3–5 and days 7–10), at 3 months and after 1 year. Levels of activation and temporal profiles during hospitalization were related to disease severity defined as respiratory failure (PO2/FiO2 ratio <26.6 kPa) and/or admission to intensive care unit, 60-day total mortality and pulmonary pathology after 3 months. Findings: During hospitalization, TCC, C4d, C3bc, C3bBbP and C5a were significantly elevated compared to healthy controls. Severely ill patients had significantly higher levels of TCC and C4d (p < 0.001), compared to patients with moderate COVID-19. Escalated levels of TCC and C4d during hospitalization were associated with a higher risk of 60-day mortality (p < 0.001), and C4d levels were additionally associated with chest CT changes at 3 months (p < 0.001). At 3 months and 1 year, we observed consistently elevated levels of most complement activation products compared to controls. Conclusion: Hospitalized COVID-19 patients display prominent and long-lasting systemic complement activation. Optimal targeting of the system may be achieved through enhanced risk stratification and closer monitoring of in-hospital changes of complement activation products.

• Mesinovic, Munib; Wong, Xin Ci; Rajahram, Giri Shan; Citarella, Barbara Wanjiru; Peariasamy, Kalaiarasu M. & Greve, Frank van Someren [Vis alle 1 671 forfattere av denne artikkelen] (2024). At-admission prediction of mortality and pulmonary embolism in an international cohort of hospitalised patients with COVID-19 using statistical and machine learning methods. Scientific Reports. 14(1). doi: 10.1038/s41598-024-63212-7. Fulltekst i vitenarkiv

Se alle arbeider i NVA

• Granerud, Beathe Kiland (2025). Profesjonsetikk - hvorfor skal vi bry oss om det? Fulltekst i vitenarkiv Vis sammendrag

  Deontologisk etikk (Kant), konsekvensetikk (utilitarismen), dydsetikk (Aristoteles), biomedisinsk etikk, dyder i helseprofesjoner, SME-modellen for etisk refleksjon.

• Granerud, Beathe Kiland (2025). Bioteknologi i praksis - erfaringer fra en bioingeniør. Fulltekst i vitenarkiv Vis sammendrag

  Bioingeniørers arbeidshverdag, real-time PCR, sekvensering.

• Granerud, Beathe Kiland (2024). From young researchers’ point of view – what works and not when setting up NGS. Fulltekst i vitenarkiv
• Stenseth, Else Berit; Wergeland, Line; Granerud, Beathe Kiland; Syversen, Linda; Andersen, Heidi & Ersvær, Elisabeth (2023). Conditions for Communication, information and collaboration Between Higher education and healthcare Institutions organising clinical placements: The Biomedical Laboratory Scientists Perspectives. Fulltekst i vitenarkiv

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